Cover Feature: Bacterial Cell‐Surface Display of Semisynthetic Cyclic Peptides (ChemBioChem 1/2019)

Puzzling Peptides from a Phage Display Library

The commercial availability of random peptide libraries displayed on the M13 phage is increasing their use forstudies on epitope identification, enzyme inhibitors, receptor ligands, etc. In this study two experimentswhere planned for selection of peptides. First with sheep antibodies, the positive selector was IgG, preparedon Protein G column from a pool of 11 sheeps immunized...

Directed evolution of a biterminal bacterial display scaffold enhances the display of diverse peptides

Bacterial cell-surface display systems coupled with quantitative screening methods offer the potential to expand protein engineering capabilities. To more fully exploit this potential, a unique bacterial surface display scaffold was engineered to display peptides more efficiently from the surface exposed C- and N-termini of a circularly permuted outer membrane protein. Using directed evolution,...

Cover Picture: Detection of mRNA in Mammalian Cells with a Split Ribozyme Reporter (ChemBioChem 6/2006)

The cover picture shows a split RNA reporter strategy for detecting target mRNA in mammalian cells. A tetrahymena group I intron ribozyme reporter is split into two halves, each of which carries a part of the coding sequence of the reporter mRNA, a fragment of the ribozyme, and an antisense sequence complementary to a target mRNA. Both halves bind with the target mRNA and are assembled into an ...

Cover Feature Outlook

Cover Feature Physiological Computing

Brain−computer interfaces (BCIs) hold great promise for improving information delivery and preserving user attention, but this promise has not yet translated to practical use. A prototype BCI that optimizes email notifications in noisy, complex environments, CARSON combines multiple measures from the brain to predict both cognitive workload and message relevancy to determine the optimum time to...